How to create burn porcien models: A systematic review

Wardhana A1, Kurniasari D1, Lumbuun R1

1 Burn Unit Division of Plastic Surgery Cipto Mangunkusumo National General Hospital, Diponegoro Road 71, Jakarta, , Indonesia, ruthlumbuun@yahoo.com

Background

Management of burn patients is challenging for every burn surgeons, it needs a lot of knowledge and experience by doing researches. However, experimental study is not morally and ethically accepted to be done in human. Therefore this study can be done in animals. Porcine is more frequently used as experimental model because from anatomically and physiologically pig skin is more similar to human skin.

Methods

We included systematic reviews, meta analysis, and experimental studies of burns which use porcine models in their studies. We excluded studies more than 10 years and included only thermal injuries. The grade of burn injury that was made to the porcine model has to be second or third degree burns. Search methods we used were from Ovid MEDLINE, Pubmed, Burns Journal, and Cochrane Library.

Result

Searches from four databases revealed 21 studies. To create full thickness burn wounds, there were 3 methods found: aluminium, brass, and immersing in hot water. With aluminium methods, the aluminium bar was heated until 200°C and placed to the pig’s skin for 20 seconds. For deep dermal burn, there are various methods: modified glass bottle and aluminium bar. The bottle is filled with sterile water and heated until the desire temperature (92°C) then placed to the pig’s skin for 15 seconds. To measure the depth of the burn wound, almost all of the study use the histopathological evaluation.

Conclusion

There was no standardised method to create burn wounds in porcine model. Nevertheless, for deep dermal burn wounds, we can use modified glass bottle method and for full thickness burn wounds, we can use aluminium or brass. There are no prior studies discuss about how to make burn porcine models and also no studies in this review focusing on created the burn wound alone. Further studies needed to achieve better results in how to create burn wounds in porcine models.

Key Words

burn, pig, burn porcine models, full thickness burn, deep dermal burn

Biography

Presenting author is a general practitioner currently works in Burn Unit Plastic Surgery Division Cipto Mangunkusumo National General Hospital as a research assistant.

About ANZBA

ANZBA is a not for profit organisation and the peak body for health professionals responsible for the care of the burn injured in Australia and New Zealand. ANZBA encourages higher standards of care through education, performance monitoring and research.

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