Prof. Ligen Li1
1First Affiliated Hospital Of Pla General Hospital, Beijing City, China
Object This experimental study aims 1) to observethe expression and distribution of NLRP3 inflammasomein burn wounds ofarat burn model; 2) to study whether inhibiting the NLRP3 inflammasome activation wouldameliorateburn wound progression.
Methods:A deep second degree burn was inflicted on the back of Wistarrats. The expression of NLRP3 inflammasomecomponents and IL-1β were determined by western blot and coimmunoprecipitation. The distribution of NLRP3 inflammasome was assessed by immunohistochemical staining and double-labelling immunofluorescence.Neutrophil infiltration, wound perfusion, burn depth and wound healing time were assessed.
Results: Burn induced remarkable NLRP3 inflammasome activation and cleavage of IL-1β. The NLRP3 inflammasome was mainly observed in macrophages of the zone of stasis. 3, 4-Methylenedioxy-β-nitrostyrene(MNS) significantly inhibited the NLRP3 inflammasome activation and inflammatory cytokines production in burn wounds. Consequently, neutrophil infiltration was reduced, wound perfusion was restored, burn wound progression was ameliorated and wound healing was accelerated.
Conclusion: In this study, we demonstrated that burn induced NLRP3 inflammasome activationand inflammatory response in wounds, which may be associated withburnwound progression. Treatment with MNS inhibited NLRP3 inflammasomeactivation, ameliorated burn wound progression and promoted wound healing.
Keywords: 3, 4-Methylenedioxy-β-nitrostyrene;burn; inflammasomes; NLRP3 protein;
Professor, First Affiliated Hospital Of PLA General Hospital,Beijing,China