Preclinical assessment of efficacy of Wharton’s jelly mesenchymal stem cells on a scaffold of amniotic membrane in wound healing using a porcine model.

Dr Susan Jehangir1, Dr  John Harvey1, Dr  Peter Maitz2, Dr Zhe Li2

1Children’s Hospital At Westmead, Westmead, Australia, 2Burns Unit, Concord Hospital, , Concord, Australia

Abstract:

Aim

This project studies the regeneration of epidermal and dermal components with the use human Wharton’s Jelly Mesenchymal Cells (WJ-MSCs) on a scaffold of Freeze Dried Human Amniotic Membrane (FD-HAM) and Cryopreserved Human Amniotic Membrane (CP-HAM) in porcine excisional wound model

Methods

Fresh AM was harvested from seronegative term pregnant mothers undergoing elective Caesarean section deliveries under sterile conditions. The membrane was de-cellularised  and either cryopreserved (CP-HAM) or freeze dried (FD-HAM) for storage. Commercially available WJ-MSCs  were seeded on the different forms of the decellularised membrane. Six, 5×5 cm cutaneous wounds using a dermatome at 0.8mm setting, 2cm apart will be created on the dorsum of 20-25kg Great White pigs on either side of the spine under general anaesthesia. The treatments (Wound control, WJ-MSCs alone, FD-HAM, CP-HAM, FD-HAM + WJ-MSCs, CP-HAM+ WJ-MSCs)   were allocated by Latin squares to randomise the application. A barrier dressing were applied. Wound healing was followed for 28 days using wound imaging and histology at euthanasia. The healing wound was assessed by Bates Jenson score and the healed wound by the Vancouver Scar scale. The final histology will assess the epithelization, sweat and hair follicle regeneration and fibrosis in the wounds.

Results

The study is in progress. Results are awaited.


Biography:

Susan is a fellow at the Children’s Hospital at Westmead  visiting as a short term trainee from Christian Medical College Vellore, India

About ANZBA

ANZBA is a not for profit organisation and the peak body for health professionals responsible for the care of the burn injured in Australia and New Zealand. ANZBA encourages higher standards of care through education, performance monitoring and research.

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